Atomic Force Microscopy

Author: Pier Carlo Braga
Publisher: Springer Science & Business Media
ISBN: 1592596479
Size: 27.31 MB
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Highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.

Principles And Techniques Of Biochemistry And Molecular Biology

Author: Keith Wilson
Publisher: Cambridge University Press
ISBN: 9780521828895
Size: 30.80 MB
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This is a new and expanded edition of the bestselling Principles and Techniques of Practical Biochemistry. It includes discussion of the theoretical principles, practical details and applications of the key experimental techniques that are routinely used in modern biochemistry and molecular biology, including those that have led to the emergence of the new disciplines of genomics, proteomics and bioinformatics. All chapters have been updated and new sections added to cover the principles of experimental design, the statistical analysis of quantitative analytical data, and the principles and practice of clinical biochemistry. here are new chapters covering cell culture, microscopy and mass spectrometry. To enhance student understanding of each topic, in-text worked examples are included in each chapter. The textbook will be essential reading for all bioscience students and pre-clinical medical students.

Romeis Mikroskopische Technik

Author: Maria Mulisch
Publisher: Springer-Verlag
ISBN: 3642551904
Size: 33.61 MB
Format: PDF
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Der ROMEIS ist seit fast 100 Jahren das Standardwerk der mikroskopischen Technik. Über 18 Auflagen hat dieses Methodenbuch die Entwicklung der lichtmikroskopischen Verfahren begleitet und ist bis heute ein unverzichtbares Laborhandbuch für Wissenschaftler und Studierende, die auf den Gebieten der Cytologie, Histologie, mikroskopischen Anatomie, Pathologie und Histochemie forschen. Der Inhalt der 19. Auflage des ROMEIS wurde aktualisiert und um viele moderne Methoden und Anwendungen der Mikroskopie erweitert. Unter der Herausgeberschaft von Privatdozentin Dr. Maria Mulisch und Professor Dr. med. Ulrich Welsch haben 24 Experten der Mikroskopie aus Forschung und Industrie ihre Erfahrung eingebracht, um dieses Werk zu einem Arbeitsbuch zu machen, auf das man sich beziehen und verlassen kann.

Scanning Probe Microscopy

Author: Sergei V. Kalinin
Publisher: Springer Science & Business Media
ISBN: 0387286683
Size: 25.26 MB
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This volume will be devoted to the technical aspects of electrical and electromechanical SPM probes and SPM imaging on the limits of resolution, thus providing technical introduction into the field. This volume will also address the fundamental physical phenomena underpinning the imaging mechanism of SPMs.

Bioimaging Current Concepts In Light Electron Microscopy

Author: Douglas E Chandler
Publisher: Jones & Bartlett Publishers
ISBN: 0763738743
Size: 80.15 MB
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The development of microscopy revolutionized the world of cell and molecular biology as we once knew it and will continue to play an important role in future discoveries. Bioimaging: Current Concepts in Light and Electron Microscopy is the optimal text for any undergraduate or graduate bioimaging course, and will serve as an important reference tool for the research scientist. This unique text covers, in great depth, both light and electron microscopy, as well as other structure and imaging techniques like x-ray crystallography and atomic force microscopy. Written in a user-friendly style and covering a broad range of topics, Bioimaging describes the state-of-the-art technologies that have powered the field to the forefront of cellular and molecular biological research. Important Notice: The digital edition of this book is missing some of the images or content found in the physical edition.

Atomic Force Microscopy In Biomedical Research

Author: Pier Carlo Braga
Publisher: Humana Press
ISBN: 9781617791048
Size: 17.12 MB
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With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.

Chemical Biology

Author: Banafshe Larijani
Publisher: John Wiley & Sons
ISBN: 0470090669
Size: 29.90 MB
Format: PDF, Kindle
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Written by a team of international researchers and teachers at the cutting edge of chemical biology research, this book provides an exciting, comprehensive introduction to a wide range of chemical and physical techniques with applications in areas as diverse as molecular biology, signal transduction, drug discovery and medicine. Techniques include: Cryo-electron microscopy, atomic force microscopy, differential scanning calorimetry in the study of lipid structures, membrane potentials and membrane probes, identification and quantification of lipids using mass spectroscopy, liquid state NMR, solid state NMR in biomembranes, molecular dynamics, two dimensional infra-red studies of biomolecules, single and two-photon fluorescence, optical tweezers, PET imaging and chemical genetics. KEY FEATURES: a unique guide to the rapidly evolving, interdisciplinary field of chemical biology. adopts a molecular structure for maximum flexibility. addresses relevant, topical chemical biological questions throughout. includes stunning illustrations. associates website with PowerPoint slides of figures within the book. Chemical Biology: Techniques and Applications provides an invaluable resource for final year undergraduate and post graduate bioscience and biomedical students and pharmaceutical researchers with an interest in this fascinating, and ever changing field.

Applied Scanning Probe Methods Iii

Author: Bharat Bhushan
Publisher: Springer Science & Business Media
ISBN: 354026910X
Size: 54.52 MB
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The Nobel Prize of 1986 on Sc- ning Tunneling Microscopy sig- led a new era in imaging. The sc- ning probes emerged as a new i- trument for imaging with a pre- sion suf?cient to delineate single atoms. At ?rst there were two – the Scanning Tunneling Microscope, or STM, and the Atomic Force Mic- scope, or AFM. The STM relies on electrons tunneling between tip and sample whereas the AFM depends on the force acting on the tip when it was placed near the sample. These were quickly followed by the - gneticForceMicroscope,MFM,and the Electrostatic Force Microscope, EFM. The MFM will image a single magnetic bit with features as small as 10nm. With the EFM one can monitor the charge of a single electron. Prof. Paul Hansma at Santa Barbara opened the door even wider when he was able to image biological objects in aqueous environments. At this point the sluice gates were opened and a multitude of different instruments appeared. There are signi?cant differences between the Scanning Probe Microscopes or SPM, and others such as the Scanning Electron Microscope or SEM. The probe microscopes do not require preparation of the sample and they operate in ambient atmosphere, whereas, the SEM must operate in a vacuum environment and the sample must be cross-sectioned to expose the proper surface. However, the SEM can record 3D image and movies, features that are not available with the scanning probes.

Techniques In Microscopy For Biomedical Applications

Author: Terje Dokland
Publisher: World Scientific
ISBN: 9812564349
Size: 72.21 MB
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The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.